Dresden University of Technology

Department of Anesthesiology and Intensive Care Medicine


Experimental Studies

Mechanical ventilation in animal models of ARDS

Small animals: rats, rabbits
Large animals: swine


  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9

Methods

Cell cultures
Purification of nucleic acids (RNA, DNA) and proteins
Conventional and real-time PCR
Western blotting
ELISA
Immunofluorescence microscopy
FACS (Fluorescence Activated Cell Sorter)
Histology
Blood gas analysis
Pulmonary perfusion measurement (Microspheres)

Modes of mechanical ventilation

Variable pressure assist ventilation (Dräger Evita XL and Dräger Infinity)
Variable pressure/volume controlled ventilation (Dräger Evita XL, Harvard Inspira ASVp)
Neurally adjust ventilator assist (Maquet Servo I)

Clinical Trials


Engineering

Development of new strategies of ventilation (variable tidal volume ventilation)
Control system design for variable ventilation modes
Measurement and advanced analysis of respiratory signals (whole body plethysmography and Fleisch pneumotachography)
Advanced analysis of haemodynamics (ECG and continuous blood pressure)
Analysis of Electrical Impedance Tomography (EIT)
Medical image analysis; Computer Tompography (CT), Positron Emission Tomography (PET), Electrical Impedance Tomography (EIT))


nVentInspira

nVentInspira is open-source software (LabVIEW script) developed to perform variable controlled tidal volume ventilation using the Harvard Inspira ASVp small animal ventilator. This controller enables compliance compensation and variation of tidal volume during volume controlled ventilation. nVentInspira is available for the interested research community.

A detailed description of the controller implemented in nVentInspira and its performance can be found here:


Huhle, Robert, Peter Spieth, Andreas Güldner, Thea Koch and Marcelo Gama de Abreu: ‘A New Adaptive Controller for Volume-Controlled Mechanical Ventilation in Small Animals’ Experimental Lung Research 40, no. 4 (May 2014): 186–97.doi:10.3109/01902148.2014.900156.

E-mail requests robert.huhle@uniklinikum-dresden.de are welcome to get a copy of nVentInspira. Please cite the reference given above if you used this software in your experimental studies.


Author: Robert Huhle

Contact

Head of Group:
Prof. Dr. med.
Marcelo Gama de Abreu
Tel.: +49 351 458-4488
Fax: +49 351 458-4336
mgabreu@
uniklinikum-dresden.de


Laboratory Coordinators
Experimental Trials:
Dr. med. Martin Scharffenberg
Tel.: +49 351 458-19734
martin.scharffenberg@
uniklinikum-dresden.de

Dr. med. Jakob Wittenstein
Tel.: +49 351 458-19887
jakob.wittenstein@
uniklinikum-dresden.de


Laboratory Coordinators
Clinical Trials:
Dr. med. Thomas Kiss
Tel.: +49 351 458-18055
thomas.kiss@
uniklinikum-dresden.de

Dr. med. Thomas Bluth
Tel.: +49 351 458-19007
thomas.bluth@
uniklinikum-dresden.de

Postal Address:
Fetscherstrasse 74
01307 Dresden

Visitor Address:
Medizinisch Theoretisches Zentrum (MTZ)
House 91
Laboratory A.20.020
Fiedlerstrasse 42
01307 Dresden